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. Author manuscript; available in PMC: 2018 Oct 25.
Published in final edited form as: J Am Chem Soc. 2018 Jul 9;140(28):8739–8745. doi: 10.1021/jacs.8b03956

Figure 6.

Figure 6.

Theophylline-regulated cellular RNA levels based on a hammerhead ribozyme (HHR) system. (a) Schematic of theophylline- induced HHR self-cleavage and target RNA release. The red triangle denotes the cleavage site of HHR. (b) In vitro fluorescence assay data after adding different concentrations of theophylline. (c) 10% denaturing PAGE gel characterization of the 10-base-long inhibitor- incorporated HHR system at 2 h after adding different concentrations of theophylline. (d) Confocal fluorescence imaging of the theophylline−HHR construct treated with different concentrations of theophylline. These BL21 (DE3)* cells were induced by IPTG for 1 h before imaging.