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Kinetic constants for the wild-type and mutant MbNHasesa
| kcat (s−1) | Km (mM) | kcat/Km (s−1mM−1) | Metal Content | |
|---|---|---|---|---|
| Wild-typeb | 131 ± 3 | 83 ± 10 | 1.6 | 1.8 ± 0.1 |
| Cleaved | 163 ± 4 | 93 ± 15 | 1.8 | 1.9 ± 0.1 |
| Δ238–257 | 71 ± 4 | 104 ± 17 | 0.7 | 1.8 ± 0.1 |
| Δ238–257 + PtNHaseact | 166 ± 5 | 125 ± 17 | 1.3 | 2.3 ± 0.2 |
| Δ219–272 | 75 ± 8 | 73 ± 14 | 1.0 | 1.7 ± 0.1 |
| Δ219–272 + PtNHaseact | 117 ± 10 | 149 ± 23 | 0.8 | 1.7 ± 0.1 |
Acrylonitrile was used as the substrate.
Reference [11].
