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. 2018 Sep 27;9:574. doi: 10.3389/fendo.2018.00574

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Copyright © 2018 Liu, He, Ning, Luo, Li, Zhang, Chen and Du.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Body composition and metabolic characteristics of DIO Nile tilapia induced by HCD or HFD feeding. (A) Body weight changes. ^#HFD vs. HCD, ^&HCD vs. ND, ^#P < 0.05, ^&P < 0.05, ^##P < 0.01 and ^&&P < 0.01 (n = 3, 120 fish per group). (B) Whole fish fat content (100% × fat weight/body weight, dry weight ratio) (n = 6). (C) Visceral adipose tissue-somatic index (VSI) (100 × visceral adipose tissue weight / body weight, wet weight ratio) (n = 6). (D) Liver TG content (n = 6). (E) Muscle glycogen content (n = 6). (F) Liver glycogen content (n = 6). (G–I) Plasma TG, glucose and insulin levels (n = 6). (J–L) H&E staining of liver, VAT and SCAT. Three column graphs in the right of pictures from top to bottom represent hepatocyte size, adipocyte size and thickness of SCAT, respectively. Scale bars, 100 μm. (M) PPARα and PPARγ mRNA levels in liver (n = 6). (N,O) GTT (n = 5) and ITT (n = 6). ^*p < 0.05 and ^**p < 0.01. Data shown as means ± SEM.