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. 2018 Oct 9;9(79):34945–34971. doi: 10.18632/oncotarget.26188

Figure 2. Under CR conditions, LCA exhibits age-related differential effects on the concentrations of several classes of cellular lipids in WT yeast.

Figure 2

WT cells were cultured in the nutrient-rich YP medium initially containing 0.2% glucose with 50 μM LCA or without it. Following extraction of lipids from cells recovered on different days of culturing, various lipid classes were identified and quantitated by mass spectrometry as described in Materials and Methods. These lipid classes included free fatty acids (FFA) (A), phosphatidic acid (PA) (B), phosphatidylserine (PS) (C), phosphatidylethanolamine (PE) (D), phosphatidylcholine (PC) (E), phosphatidylinositol (PI) (F), phosphatidylglycerol (PG) (G), cardiolipin (CL) (H), monolysocardiolipin (monolysocardiolipin) (I) and triacylglycerol (TAG) (J). Data are presented as means ± SEM (n = 3; *p < 0.05; **p < 0.01; ns, not significant). Abbreviations: CL, cardiolipin; FFA, free fatty acids; L, D, PD and ST, logarithmic, diauxic, post-diauxic and stationary growth phases (respectively); MLCL, monolysocardiolipin; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; TAG, triacylglycerol.