Table 2. Yield of RNA by different homogenization methods.
| Tissue type | Set screw | TissueLyser | Bullet blender | Pellet pestle | Dounce |
|---|---|---|---|---|---|
| Retina |
4605 ± 1076 |
8345 ± 1450 |
5600 ± 481 |
2245 ± 452 |
7380 ± 994 |
| Whole eye | 7724 ± 2753 | 9784 ± 1779 | 8347 ± 834 | 2982 ± 1001 | 4584 ± 2103 |
Values are mean total ng in 40 μl eluent ± SD, n=8 for all retina measurements, n=10 for whole eyes except the pellet pestle (n=9). ANOVA multiple comparison analysis compared the mean of each technique within a tissue type (retina or whole eye) with the mean of every other technique for the same tissue. A full list of comparisons with p values is given in Appendix 1 and Appendix 2.