Table 4. RNA quality by mean RIN score by different homogenization methods ± SD.
| Tissue type | Set screw | TissueLyser | Bullet blender | Pellet pestle | Dounce |
|---|---|---|---|---|---|
| Retina |
8.33 ± 0.47 |
7.75 ± 0.64 |
8.36 ± 0.63 |
7.91 ± 0.64 |
8.19 ± 0.81 |
| Whole eye | 8.28 ± 0.20 | 8.18 ± 0.25 | 8.78 ± 0.18 | 8.71 ± 0.42 | 8.65 ± 0.35 |
Values are mean RIN scores ± SD n=8 for all retina measurements, n=10 for whole eyes except the pellet pestle (n=9). ANOVA multiple comparison analysis compared the mean of each technique within a tissue type (retina or whole eye) with the mean of every other technique for the same tissue. A full list of comparisons with p values is given in Appendix 5 and Appendix 6.