Figure 9.

Influence of single-amino acid substitutions on the overall structure of the GIIβ MRH domain. Two-dimensional ¹⁵N–¹H HSQC spectra of the wild type and four mutants containing a single-amino acid substitution in the binding pocket. (A) Overlay of four point mutants and wild-type protein in the absence of Man-6-P. (B) Overlay of spectra of the wild-type protein in the absence (black) and presence of 200 mM Man-6-P (pink). (C) Overlay of spectra of the GIIβ-MRH mutants in the absence and presence of 200 mM Man-6-P.