Key resources table.
| Reagent type (species) or resource | Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | wgGBM | this paper | NA | This allele carries the point mutation of K234E and M238R in Wg |
| Genetic reagent (D. melanogaster) | wg1 | doi: 10.1523/Jneurosci.3714–13.2014 | RRID: BDSC_2978 | |
| Genetic reagent (D. melanogaster) | wgCX4,wgl-17 | doi: 10.1016/S0092-8674 (02)01047–4 | RRID: BDSC_2980 | |
| Genetic reagent (D. melanogaster) | UAS-Wg-HA(6C) | doi: 10.1016/S0092-8674 (02)01047–4 | RRID: BDSC_5918 | |
| Genetic reagent (D. melanogaster) | UAS-Wg-HA(3C) | doi: 10.1016/S0092-8674 (02)01047–4 | RRID: DGGR_108488 | |
| Genetic reagent (D. melanogaster) | egh7 | PMID: 9012507 | RRID: BDSC_3902 | |
| Genetic reagent (D. melanogaster) | egh62d18 | PMID: 9012507 | NA | Dr. Stephen M. Cohen (National University of Singapore) |
| Genetic reagent (D. melanogaster) | UAS-Egh-Myc | doi: 10.1074/jbc.C400571200 | NA | |
| Genetic reagent (D. melanogaster) | brnfs107 | PMID: 1483386 | RRID: BDSC_4303 | |
| Genetic reagent (D. melanogaster) | brn1.6P6 | PMID: 1483386 | RRID: BDSC_50762 | |
| Genetic reagent (D. melanogaster) | UAS-Brn | doi: 10.1016/j.ydbio.2007.04.013 | NA | |
| Genetic reagent (D. melanogaster) | UAS-brn-RNAi | doi: 10.1038/nmeth | RRID: BDSC_55386 | |
| Genetic reagent (D. melanogaster) | arrk08131 | doi: 10.1534/genetics.104.026427 | RRID: BDSC_665 | |
| Genetic reagent (D. melanogaster) | arr2 | doi: 10.1038/35035110 | RRID: BDSC_3087 | |
| Genetic reagent (D. melanogaster) | evi2 | doi: 10.1074/jbc.M112.342667 | NA | Dr. Vivian Budnik (University Massachusetts Medical School) |
| Genetic reagent (D. melanogaster) | lace2 | doi: 10.1128/MCB.19.10.7276 | RRID: BDSC_3159 | |
| Genetic reagent (D. melanogaster) | lacek05305 | doi: 10.1128/MCB.19.10.7276 | RRID: BDSC_12176 | |
| Genetic reagent (D. melanogaster) | Df(2L)Exel7063 | NA | RRID:BDSC_7831 | |
| Genetic reagent (D. melanogaster) | schlankG0489 | doi:10.1038/emboj.2009.305 | RRID: DGGR_111936 | |
| Genetic reagent (D. melanogaster) | schlankG0061 | doi:10.1038/emboj.2009.305 | RRID: BDSC_11665 | |
| Genetic reagent (D. melanogaster) | Sk2KG05894 | doi: 10.1194/jlr.M300005-JLR200 | RRID: BDSC_14133 | |
| Genetic reagent (D. melanogaster) | Df(3L)BSC671 | NA | RRID: BDSC_26523 | |
| Genetic reagent (D. melanogaster) | UAS-ManII-GFP | doi:10.1016/j.cell.2007.06.032 | RRID: BDSC_65248 | Dr. Yuh Nung Jan (University of California, San Francisco) |
| Genetic reagent (D. melanogaster) | UAS-Spin-GFP | doi: S0896627302010140 | RRID:BDSC_39668 | Dr. Graeme Davis (University of California, San Francisco) |
| Genetic reagent (D. melanogaster) | UAS-YFP-Rab5 | doi: 10.1534/genetics.106.066761 | RRID: BDSC_24616 | |
| Genetic reagent (D. melanogaster) | nSyb-Gal4 | NA | RRID: BDSC_51635 | |
| Genetic reagent (D. melanogaster) | OK6-Gal4 | NA | RRID: BDSC_64199 | |
| Genetic reagent (D. melanogaster) | C57-Gal4 | NA | RRID: BDSC_32556 | |
| Genetic reagent (D. melanogaster) | Repo-Gal4 | NA | RRID: BDSC_7415 | |
| Genetic reagent (D. melanogaster) | act-Cas9 | NA | RRID: BDSC_54590 | |
| Antibody | Mouse IgM anti-MacCer | doi: 10.1074/jbc.C400571200 | NA | IHC (1:1) |
| Antibody | Mouse anti-CSP | DSHB Cat. #: 6D6 | RRID:AB_528183 | IHC (1:300) |
| Antibody | Mouse anti-Wg | DSHB Cat. #: 4D4 | RRID:AB_528512 | IHC (1:10), WB (1:50) |
| Antibody | Mouse anti-Futsch | DSHB Cat. #: 22C10 | RRID:AB_528403 | IHC (1:50) |
| Antibody | Mouse anti-DLG | DSHB Cat. #: 4F3 | RRID:AB_528203 | IHC (1:500) |
| Antibody | Mouse anti-GluRIIA | DSHB Cat. #: 8B4D2 | RRID:AB_528269 | IHC (1:200) |
| Antibody | Mouse anti-Fas II | DSHB Cat. #: 1D4 | RRID:AB_528235 | IHC (1:50) |
| Antibody | Mouse anti-Syx1A | DSHB Cat. #: 8C3 | RRID:AB_528484 | IHC (1:20), WB (1:1000) |
| Antibody | Mouse anti-dFz2 | DSHB Cat. #:12A7 | RRID:AB_528257 | IHC (1:5) |
| Antibody | Mouse anti-Rab11 | BD Biosciences Cat. #: 610656 | RRID:AB_397983 | IHC (1:50) |
| Antibody | Mouse anti-HA | MBL International Cat. #: M180 | RRID:AB_10951811 | IHC (1:1000) |
| Antibody | Mouse anti-Myc | CWBIO Cat. #: CW0259 | IHC (1:300), WB (1:1000) | |
| Antibody | Mouse anti-Myc | MBL International Cat. #: M192 | RRID:AB_11160947 | IHC (1:300), WB (1:1000) |
| Antibody | Rat anti-GFP | MBL International Cat. #: D153 | RRID:AB_591820 | IHC (1:200) |
| Antibody | fluorescence-conjugated anti-HRP | Jackson ImmunoResearch | RRID:AB_2314647 | IHC (1:200) |
| Antibody | Mouse anti-β-actin | Millipore Bioscience Research | RRID:AB_2223041 | WB (1:50000) |
| Chemical compound, drug | D,L-threo-PDMP | Matreya | Cat.#: 1719 | 0.5 mg/ml |
| Chemical compound, drug | filipin III | Cayman | 70440 | 50 μg/ml |
| Chemical compound, drug | MβCD | Sigma-Aldrich | C4555 | 20 mM |
| Chemical compound, drug | Lactosylceramide | Matreya | Cat.#: 1500 | |
| Peptide, recombinant protein | GBM | this paper | CKCHGMSGSCTVKTC | |
| Peptide, recombinant protein | GBMmut | this paper | CECHGRSGSCTVKTC | |
| Recombinant DNA reagent | pcDNA3.1-Myc-Wg | Invitrogen | V790-20 | |
| Recombinant DNA reagent | U6b-sgRNA | Addgene | 65956 | |
| Recombinant DNA reagent | pBluescript SK (-) | Stratagene | 212206 | |
| Commercial assay or kit | TNT T6 Quick Coupled Transcription/Translation System |
Promega | Cat.#: L1171 | |
| Commercial assay or kit | LacCer-coated beads | Echelon | Cat.#: P-B0LC | |
| Commercial assay or kit | Control beads | Echelon | Cat.#: P-B000 | |
| Commercial assay or kit | HPTLC Silica Gel (aluminium plates) | Merck-Millipore | Cat.#: 105547 | |
| Software, algorithm | Hyperchem software | Hypercube |