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. Author manuscript; available in PMC: 2018 Oct 25.
Published in final edited form as: Methods Mol Biol. 2018;1684:129–150. doi: 10.1007/978-1-4939-7362-0_11

Figure 2:

Figure 2:

Proteoliposome reconstitution and patched membrane stretching. (A) Photograph of dehydrated/rehydrated proteoliposomes in a 35 mm glass bottom dish prior to addition of bath solution. Seven, three-microliter drops of proteoliposomes were dehydrated prior to rehydration overnight with 20 μL DR buffer. (B) A pipette tip positioned next to the filament of a microforge (left) prior to and (right) after application of heat to bend the tip to a ~35° angle. (D) A bent pipette tip positioned adjacent to a group of unilamellar proteoliposomes to be patched. (E) A bent pipette tip with patched membrane. The radius of curvature of the membrane can be fit and used with the recorded pressure difference to calculate tension within the membrane.