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. 2018 Oct 2;42(6):3291–3299. doi: 10.3892/ijmm.2018.3913

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Copyright: © Lu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of CYC1 on SCC9 cells. At 48 h after cell transfection with lncRNA C5orf66-AS1-siRNA or lncRNA C5orf66-AS1-siRNA + CYC1-siRNA, the (A) proliferation, (B) apoptosis, (C) migration and (D) invasion of SCC9 cells were determined using an MTT assay, flow cytometry, wound healing assay and transwell assay, respectively. Wound closure was quantified and the number of invasive cells was counted. ^**P<0.01 vs. control groups. Magnification, ×100 (migration) and ×200 (invasion). lncRNA, long non-coding RNAs; C5orf66-AS1, C5orf66 antisense RNA 1; siRNA, small interfering RNA; NC, negative control; CYC1, cytochrome c1.