Table III.
Mean methylation values and medians of the MI and each CpG site in KLF4 and HS3ST2 genes according to diagnosis.
| Site | Normal (n=43) | Hyperplasia (n=35) | Cancer (n=91) | P-value |
|---|---|---|---|---|
| MI KLF4a | 23.83±11.39 | 30.40±8.52 | 34.72±10.79 | |
| 25.33 | 29.50 | 31.83 | 0.001 | |
| CpG1 | 32.65±18.77 | 40.49±17.07 | 50.22±17.42 | |
| 32.00 | 36.00 | 47.00 | <0.001 | |
| CpG2 | 22.51±9.96 | 29.69±8.29 | 31.91±9.80 | |
| 23.00 | 29.00 | 30.00 | <0.001 | |
| CpG3 | 23.05±10.05 | 28.14±7.69 | 32.76±10.68 | |
| 24.00 | 26.00 | 30.00 | <0.001 | |
| CpG4 | 20.30±10.01 | 25.69±8.23 | 29.78±9.31 | |
| 22.00 | 24.00 | 28.00 | <0.001 | |
| CpG5 | 22.79±10.01 | 28.09±8.05 | 31.85±9.52 | |
| 23.00 | 27.00 | 29.00 | <0.001 | |
| CpG6 | 21.70±10.32 | 30.34±7.35 | 31.80±10.47 | |
| 24.00 | 29.00 | 29.00 | <0.001 | |
| MI HS3ST2a | 8.52±2.57 | 33.76±20.66 | 34.49±18.39 | |
| 8.33 | 36.67 | 35.00 | <0.001 | |
| CpG1 | 6.21±2.63 | 10.43±7.11 | 21.59±15.35 | |
| 6.00 | 6.00 | 21.00 | <0.001 | |
| CpG2 | 11.33±3.26 | 54.77±36.14 | 43.88±21.81 | |
| 11.00 | 60.00 | 45.00 | <0.001 | |
| CpG3 | 8.02±2.76 | 36.09±21.96 | 38.00±20.26 | |
| 7.00 | 41.00 | 40.00 | <0.001 |
a
MI was calculated as mean methylation value of analysed CpG sites; values are expressed as the mean ± standard deviation and median. MI, methylation index; KLF4, kruppel like factor 4; HS3ST2, heparan sulfate-glucosamine 3-sulfotransferase 2.