Skip to main content
. 2018 Oct 9;42(6):3181–3192. doi: 10.3892/ijmm.2018.3921

Figure 1.

Figure 1

Hypoxia promotes YAP activation in growth plate chondrocytes via a Hippo-independent signaling pathway. (A) Protein expression of MST1, p-LATS, LATS, p-YAP and YAP of growth plate chondrocytes cultured under hypoxia (1% O2) for 0, 2, 4 and 8 h. β-actin served as an internal control. (B) The density of the bands were quantified and normalized to the control group (0 h). (C) mRNA expression of MST1, LATS1, LATS2, YAP and CTGF in chondrocytes cultured under hypoxia (1% O2) for 0, 2, 4 and 8 h. (D) Immunofluorescence imaging of YAP (red) in chondrocytes cultured under normoxia (21% O2) or hypoxia (1% O2) for 4 h. DAPI (blue) was used for nuclear staining. Scale bar=200 μm. Data are presented as mean ± standard deviation (n=3) and normalized to the control group (0 h). *P<0.05, **P<0.01 and ***P<0.001 vs. the control group (0 h). YAP, Yes-associated protein; MST1, mammalian Ste20; LATS1/2, serine/threonine protein kinase large tumor suppressor 1/2; p, phosphorylated; CTGF, connective tissue growth factor; NO, normoxia; HY, hypoxia; RFP, red fluorescent protein.