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. 2018 Sep 21;42(6):3115–3124. doi: 10.3892/ijmm.2018.3891

Figure 2.

Figure 2

miR-1307-3p inhibits the chondrogenic differentiation of hADSCs. hADSCs were infected with recombinant lentivirus expressing miR-1307-3p or miR-1307-3p inhibitor or control. Following infection, chondrogenic differentiation was induced for 21 days. (A) Infection efficiency was determined by microscopic examination of the GFP-expressing cells (magnification, x10). (B) expression of miR-1307-3p was determined with reverse transcription-quantitative polymerase chain reaction analysis. (C) Deposition of cartilage matrix proteoglycans was determined by Alcian blue staining (magnification, x40). (D) mRNA levels of chondrogenic genes (SOX9, COL2A1 and aggrecan) were analyzed by RT-qPCR analysis. (E) Protein levels of SOX9, COL2A1 and aggrecan were detected with western blot analysis. *P<0.05, **P<0.01 and ***P<0.001. miR, microRNA; hADSCs, human adipose-derived stem cells; SOX9, sex determining region Y-box 9; COL2A1, collagen type II α1 chain; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NC, negative control.