Figure 8.

A combination of APS and HCPT is most effective at inhibiting the migration and invasion of H1299 cells. (A) Migration and (B) invasion assays were performed following treatment with 0.1 mg/ml APS, 0.1 µM HCPT or the combined agents using a Transwell assay with and without Matrigel. Photomicrographs and bar diagrams of the number of cells that (C) migrated and (D) invaded through the filter to the fibronectin-coated surface following crystal violet staining. Images were captured using an optical microscope at a ×40 magnification. Data are presented as the mean ± standard error of the mean of three independent experiments with 10 random fields counted for each chamber. Scale bar, 200 µM. APS, Astragalus polysaccharide; HCPT, 10-hydroxycamptothecin. ^**P<0.01 vs. control.