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. 2018 Sep 7;42(6):3278–3290. doi: 10.3892/ijmm.2018.3866

Figure 5.

Figure 5

SNAP29 is modified by O-GlcNAc and form a complex with STX17 and VAMP8. In heart tissues from vehicle and streptozotocin-induced rats, the interaction between the O-GlcNAc modification and SNAP29-VAMP8-STX17 was evaluated by co-IP. (A) O-GlcNAc antibody was used as the known target to pull down the complex proteins SNAP29, VAMP8 and STX17, and Tubulin was used as the loading control. (B) Similarly, SNAP29 antibody was used as pull down the O-GlcNAc, VAMP8 and STX17, SNAP29 and Tubulin were used as the loading control; (C) VAMP8 antibody was applied to pull down the complex SNAP29 and STX17, VAMP8 and Tubulin were used as the loading control. (D) STX17 antibody was used to pull down the complex SANP29 and VAMP8, both STX17 and Tubulin acted as the loading control. (*P<0.05, **P<0.01, ***P<0.001 vs. vehicle group). O-GlcNAc, O-linked β-N-acetylglucosamine; SNAP29, synaptosomal-associated protein 29; vesicle-associated membrane protein 8; STX17, syntaxin-17; Ctrl, control; co-IP, coimmunoprecipitation.