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. Author manuscript; available in PMC: 2019 Aug 4.
Published in final edited form as: J Cell Physiol. 2018 Aug 4;234(1):369–381. doi: 10.1002/jcp.26930

Figure 1.

Figure 1

Expression of miR-638 is downregulated in proliferative ASMCs. Human ASMCs were seeded into 6-well plates and cultured in complete medium for 24 h at 40~60% confluence. Starvation was perform with 0.5% FBS medium and then treated with PDGF-BB (20 ng/mL) or 10% FBS. A, PDGF-BB induced a time-dependent decrease of miR-638 expression in ASMCs as determined by qRT-PCR (n=4). *p < 0.05 compared with the time at 0 h group. B, PDGF-BB induced a decreased expression of miR-638 in a dose-dependent manner at 24 h in ASMCs as determined by qRT-PCR (n=4). *p < 0.05 compared with the dose of 0 ng/mL group. C, FBS decreased expression of miR-638 in human ASMCs. Starved ASMCs were treated with 10% FBS for the indicated times and expression of miR-638 was determined by qRT-PCR (n=4). *p < 0.05 compared with the time at 0 h group. D, human ASMCs were treated with various mitogens for 24 h and expression of miR-638 was determined by qRT-PCR (n=4). miR-638 expression was significantly inhibited by PDGF-BB (20 ng/mL) and TGF-β1 (20 ng/mL), but not by other stimuli, such as PGE2 (20 ng/mL) and ET-1 (20 ng/mL). *p < 0.05 compared with vehicle.