miR-638 knockdown enhances ASMC proliferation. A, human ASMCs were transfected with either anti-miR-638 or control miR (100 nM). 72 h after transfection, miR-638 expression was determined by qRT-PCR (n=4). *p < 0.05 compared with control miR group. B, human ASMCs were transfected with either Anti-miR-638 or control miR (100 nM). 24 h after transfection, cells were starved for 48 h then stimulated with or without PDGF-BB (20 ng/mL) for 48 h, relative proliferation was determined by WST-8 proliferation assay (n=6). C, cultured human ASMCs were transfected with either anti-miR-638 or control miR (100 nM). 48 h after transduction, expression of PCNA was determined by western blot (n=4). *p < 0.05 compared with control miR group.