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. Author manuscript; available in PMC: 2019 Aug 4.
Published in final edited form as: J Cell Physiol. 2018 Aug 4;234(1):369–381. doi: 10.1002/jcp.26930

Figure 5.

Figure 5

Overexpression of miR-638 inhibits ASMC migration. A, human ASMCs were seeded into 6-well plates and transduced with either Ad-miR-638 or Ad-LacZ (100 MOI). 24 h after transduction, cells were starved for 48 h, then scratched and stimulated with or without PDGF-BB (20 ng/mL), migration was monitored by cells within the wound area at 0 h and 24 h. Migrated cells were quantitated using ImageJ software program and the result was shown in (B). n=4. C, human ASMCs were seeded into 6-well plates and transfected with either anti-miR-638 or control miR (100 nM). 24 h after transfection, starvation was performed for 48 h, then cells were scratched and stimulated with or without PDGF-BB (20 ng/mL), migration was monitored by cells within the wound area at 0 h and 24 h. Migrated cells were quantitated using ImageJ software program as shown in (D). n=4.