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. Author manuscript; available in PMC: 2019 Aug 4.
Published in final edited form as: J Cell Physiol. 2018 Aug 4;234(1):369–381. doi: 10.1002/jcp.26930

Figure 8.

Figure 8

NOR1 is critically implicated in ASMC migration. A, human ASMCs were transduced with either Ad-miR-638 or Ad-LacZ (100 MOI) for 24 h and then serum-deprived for 48 h. 3h after treated with PDGF-BB (20 ng/mL) or vehicle, NOR1 mRNA was measured by qRT-PCR (n=4). B, 6 h after treated with PDGF-BB (20 ng/mL) or vehicle, expression of NOR1 protein was detected by western blot (n=4). C, human ASMCs were infected with either Ad-NOR1 or Ad-LacZ (100 MOI) for 48 h, expression of NOR1 was detected by western blot (n=3). D, migration of human ASMCs was detected by scratch wound assay. Cells were seeded into 6-well plates and then transduced with adenovirus (100 MOI) as indicated. 24 h after transduction, cells were starved for 48 h, then scratched and stimulated with or without PDGF-BB (20 ng/mL). Migration was monitored by cell numbers within the wound area at 0 h and 24 h. Migrated cells were quantitated using ImageJ software program and quantitative results were shown in (E). n=4.