Primers used in quantitative RT-PCR |
18S rRNA-F |
GTAACCCGTTGAACCCCATT |
18S rRNA-R |
CCATCCAATCGGTAGTAGCG |
U6-F |
CTCGCTTCGGCAGCACA |
U6-R |
AACGCTTCACGAATTTGCGT |
hsa-miR-638 |
AGGGAUCGCGGGCGGGUGGCGGCCU |
cyclin D1-F |
CCTCGGTGTCCTACTTCAAATG |
cyclin D1-R |
CACTTCTGTTCCTCGCAGAC |
NOR1-F |
ATAGTCTGAAAGGGAGGAGAGG |
NOR1-R |
ATCATGCAGATTGGAGGAGAAG |
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Primers used in plasmid construction |
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Cyclin D1-3′-UTR-F |
GAGATCTAGATTGCTGCTATTGGAGGAT |
Cyclin D1-3′-UTR-R |
GAGACTCGAGCCATAGCTACACGCAAAG |
Cyclin D1-3′UTR-MUT-1F |
GACACGCGGGCGCAGTCCCACACAGGCT |
Cyclin D1-3′UTR-MUT-1R |
AGCCTGTGTGGGACTGCGCCCGCGTGTC |
Cyclin D1-3′UTR-MUT-2F |
CGCGGGCGCAGTGGCACACAGGCTGG |
Cyclin D1-3′UTR-MUT-2R |
CCAGCCTGTGTGCCACTGCGCCCGCG |