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. Author manuscript; available in PMC: 2019 Aug 4.
Published in final edited form as: J Cell Physiol. 2018 Aug 4;234(1):369–381. doi: 10.1002/jcp.26930

TABLE 1.

Primers used in this study

Name Sequence (5′-3′)
Primers used in quantitative RT-PCR
18S rRNA-F GTAACCCGTTGAACCCCATT
18S rRNA-R CCATCCAATCGGTAGTAGCG
U6-F CTCGCTTCGGCAGCACA
U6-R AACGCTTCACGAATTTGCGT
hsa-miR-638 AGGGAUCGCGGGCGGGUGGCGGCCU
cyclin D1-F CCTCGGTGTCCTACTTCAAATG
cyclin D1-R CACTTCTGTTCCTCGCAGAC
NOR1-F ATAGTCTGAAAGGGAGGAGAGG
NOR1-R ATCATGCAGATTGGAGGAGAAG

Primers used in plasmid construction

Cyclin D1-3′-UTR-F GAGATCTAGATTGCTGCTATTGGAGGAT
Cyclin D1-3′-UTR-R GAGACTCGAGCCATAGCTACACGCAAAG
Cyclin D1-3′UTR-MUT-1F GACACGCGGGCGCAGTCCCACACAGGCT
Cyclin D1-3′UTR-MUT-1R AGCCTGTGTGGGACTGCGCCCGCGTGTC
Cyclin D1-3′UTR-MUT-2F CGCGGGCGCAGTGGCACACAGGCTGG
Cyclin D1-3′UTR-MUT-2R CCAGCCTGTGTGCCACTGCGCCCGCG