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. Author manuscript; available in PMC: 2019 Oct 24.
Published in final edited form as: Cell Syst. 2018 Sep 19;7(4):384–397.e6. doi: 10.1016/j.cels.2018.08.002

Figure 4: Slowed nuclear export can cause apparent attenuation of nuclear-vs-cytoplasmic RNA noise by amplifying nuclear RNA noise and not decreasing cytoplasmic RNA noise. See also Figure S4.

Figure 4:

(A) Simulations predict that slowing the nuclear export rate shifts the nuclear-to-cytoplasmic noise ratio by affecting nuclear noise.

(B–C) smFISH analysis of HIV LTR–expressed mRNA in isoclonal cells treated with the nuclear-export inhibitor leptomycin B (green). Nuclear mean and noise increase whereas cytoplasmic mean or noise remain unchanged (grey).

(D) Comparison of nuclear versus cytoplasmic mRNA noise by smFISH analysis before and after leptomycin B treatment. All isoclonal populations remain in the amplification regime.

(E) Protein (d2GFP) noise of the same isoclones measured by flow cytometry before and after 5 hours of leptomycin B treatment. As predicted, no change in noise is observed. Inset: cytoplasmic mRNA noise from (C) for comparison. All data points represent means of two biological replicates, and error bars represent SEM.