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. Author manuscript; available in PMC: 2018 Nov 16.
Published in final edited form as: Nat Chem Biol. 2018 May 16;14(6):609–617. doi: 10.1038/s41589-018-0060-1

Fig. 1. Identification of serine hydrolases and inhibitors in live S. aureus by competitive ABPP.

Fig. 1

a) Schematic of serine hydrolase labeling in live S. aureus with FP-TMR (red star) followed by SDS-PAGE analysis to detected labeled targets. b) Schematic overview of competitive activity-based profiling platform to identify selective inhibitors of individual hydrolases from a library of small molecules (hexagons). c) Chemical structure of JCP251 and SDS-PAGE analysis of S. aureus lysates after live cells were incubated with JCP251 prior to FP-TMR labeling. Arrowheads indicate consistently observed serine hydrolase activities. d) Chemical structure of JCP678 and its FP-TMR competition labeling profile in S. aureus. Experiments shown in c,d were repeated twice with similar results.