Skip to main content
. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2018 Aug;38(8):1806–1820. doi: 10.1161/ATVBAHA.117.310663

Fig. 5. Dnm3os interacts with key nuclear proteins in macrophages.

Fig. 5

A. Gel image showing Simply Blue safe staining of proteins associated with biotinylated Dnm3os sense and control antisense RNAs using nuclear lysates from PMs. Proteins from both lanes were analyzed by mass spectrometry. B. List of top proteins associated only with Dnm3os sense RNA. C–D. Images of RNA pulldown followed by western blotting with nucleolin and ILF-2 antibodies. E–F. RNA FISH-coupled with immunofluorescence using Dnm3os probe (green color) and nucleolin or ILF-2 antibodies (red color) in Control and PA treated RAW macrophages. Scale bar 7 μm in all panels, except in enlarged images scale bars which are 4 μm. Arrows indicate location of Dnm3os.