Table 2. Biochemical characterizations of scFv 3A2 wt and isolated variants.

Standard deviations were calculated from three independent measurements.

scFv	kon (1/Ms)	koff (1/s)	KD (nM)	Potency (nM)	Inhibition type	In vitro half-life (h)
3A2	1.9×105	4.9×10−3	25 ± 2.1	39 ± 4.2	Competitive	1.0 ± 0.2
B1	7.9×105	3.9×10−3	4.9 ± 1.7	150 ± 3.5	Competitive	9.0 ± 0.3
B3	3.5×105	2.2×10−3	6.3 ± 2.4	41 ± 1.9	Competitive	7.5 ± 0.5
T1	2.0×105	4.9×10−4	2.5 ± 1.3	79 ± 4.1	Competitive	6.8 ± 0.4
T3	6.2×104	2.4×10−3	39 ± 4.6	572 ± 3.8	Competitive	11.0 ± 0.1
T4	5.3×104	4.0×10−3	75 ± 3.8	1323 ± 5.3	Competitive	6.2 ± 0.6