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. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Circulation. 2018 Sep 18;138(12):1224–1235. doi: 10.1161/CIRCULATIONAHA.118.035420

Figure 1. Severity of fibrosis varies across different mouse strains (C57BL/6J, C3H/HeJ, KK/HIJ) in response to ISO treatment.

Figure 1

(A) Wide variation in percentage of fibrosis was observed between the strains of the hybrid mouse diversity panel (HMDP) after ISO treatment. (B) Masson’s Trichrome stained sections of hearts from the three selected strains after 21 days of saline/ISO treatment (n=7–8 mice per condition). (C) Quantification of fibrotic area as a percentage of total section area (n=10–12 sections per heart). (D) Left ventricular ejection fractions (EF) were measured by echocardiography in both saline- and ISO-treated groups across the different strains at day 0 and day 21 of treatment. Changes in ejection fraction between these two timepoints were determined as the ΔEF for each mouse (n=20 mice per strain). Data presented as mean ± SEM. Two-way ANOVA, *P < 0.05. Scale bar: 1mm