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. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Circulation. 2018 Sep 18;138(12):1224–1235. doi: 10.1161/CIRCULATIONAHA.118.035420

Figure 3. CFbs display a distinct pattern of activation and proliferation that is strain-specific after in vivo ISO treatment.

Figure 3

(A) Heart sections from different strains after treatment with saline or ISO stained for αSMA (red) and Col1 (green) show presence of activated fibroblasts in perivascular and interstitial fibrotic areas. (B) Quantification of Col1+αSMA+ co-localized areas as a percentage of total heart section area (n=3–4 hearts per strain/condition). (C) Schematic of in vivo bromodeoxyuridine (BrdU) administration. (D) IHC of heart sections from different strains after treatment with saline or ISO shows BrdU+ cells within Col1+ perivascular and interstitial fibrotic regions. (E) The extent of CFb proliferation is depicted as the percentage of BrdU+ CFbs within the entire Thy+HE− population in each strain after saline or ISO treatment (n=12/strain/condition). DAPI was used to stain nuclei. Data presented as mean ± SEM. Two-way ANOVA, *P< 0.05. Scale bar: 50μm