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. Author manuscript; available in PMC: 2018 Dec 18.
Published in final edited form as: Nat Immunol. 2018 Jun 18;19(7):733–741. doi: 10.1038/s41590-018-0131-1

Fig. 4. Mutation of the PIPRSP motif in LAT impedes TCR signal transduction of DP thymocytes.

Fig. 4

(a) Flow cytometric analysis of calcium-sensitive fluorescence changes of CD45.2+mCherry+ DP T cells, loaded with Indo-I AM and stimulated with crosslinked anti-CD3 over time. (b) Flow cytometric analysis of phosphorylated Erk in CD45.2+mCherry+ DP thymocytes, in response to crosslinked anti-CD3 stimulation. (c) Flow cytometric analysis of surface expression of the TCR (left) or CD5 (right) in CD45.2+ mCherry+ CD4SP or CD8SP thymocytes. Experiments were repeated independently twice with four independent animals and yielded similar results.