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. Author manuscript; available in PMC: 2018 Oct 26.
Published in final edited form as: Leukemia. 2018 Apr 25;32(11):2339–2351. doi: 10.1038/s41375-018-0141-x

Figure 6. UFD1 inactivation in human T-ALL cells exacerbates ER stress, decreases cell growth, and induces apoptosis.

Figure 6

(a) Growth curve of human JURKAT cells was analyzed after transducing with either a control Luciferase shRNA or two UFD1 shRNA hairpins. The insert in panel (a) revealed that the two shUFD1 hairpins decreased about half of the UFD1 protein levels at day 4 postinfection. The right panel illustrates relative growth rate of human JURKAT, MOLT3 and PEER T-ALL cells. (b) Percentage of apoptotic cells upon UFD1 knockdown at day 9 or 10 postinfection in the above three cell lines. Annexin-V staining was performed on cells isolated day 4, 6 and 9/10 postinfection, and apoptosis was detected as early as day 4 postinfection. (c) Genetic knockdown of UFD1 induces moderate cell cycle changes in human JURKAT cells. At day 3 postinfection, cells were fixed and then stained with PI and analyzed by flow cytometry. Representative data from over three independent experimental repeats are shown.