Table 1.
Collection of Crystallographic Data and Refinement Statistics
| Complex | Eco RNAP – KglA | Tth RNAP – KglA |
|---|---|---|
| PDB code | 6CUX | 6CUU |
| Data Collection | ||
| Space group | P212121 | C2 |
| Cell dimensions | ||
| a (Å) | 188.167 | 185.221 |
| b (Å) | 204.685 | 101.315 |
| c (Å) | 311.577 | 294.279 |
| β (°) | 90 | 98.886 |
| Resolution (Å) | 50.0–4.1 | 50.0–3.0 |
| Total reflections | 608,898 | 358,319 |
| Unique reflections | 93,684 | 107,473 |
| Redundancy | 6.5 (6.2) | 3.3 (3.0) |
| Completeness (%)a | 99.9 (100.0) | 99.7 (97.1) |
| I / σa | 16.2 (1.95) | 10.2 (1.14) |
| Rsym (%)a | 7.7 (85.1) | 9.6 (61.4) |
| CC1/2a | (0.633) | (0.629) |
| Refinement | ||
| Resolution (Å) | 45.03–4.1 | 46.3–3.0 |
| Rwork | 0.2100 | 0.2012 |
| Rfree | 0.2547 | 0.2483 |
| No. of atoms | 54,867 | 28,254 |
| RMSDs | ||
| Bond length (Å) | 0.013 | 0.006 |
| Bond angles (°) | 1.776 | 1.089 |
| Clashscore | 11.47 | 5.84 |
| Ramachandran favored, % | 91.57 | 97.64 |
| Ramachandran outliers, % | 0.94 | 0.35 |
RMSD, root-mean-square deviation.
a
Highest-resolution shells are shown in parentheses.