REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
BL21 (DE3) for protein overexpression	ATCC	BL21 (DE3)
Chemicals, Peptides, and Recombinant Proteins
RNase E (1-529)	This study	RNase E (1-529)
RNase E (1-511)	This study	RNase E (1-511)
RNase E (1-529) R3Q, Q22D, H268S, Y269F, Q270D, K433N, R488Q, R490Q	This study	RNase E (1-529) 8x mutant
RNase E (1-529) D26N, D28N, D338N	This study	RNase E (1-529) D26N, D28N, D338N
RNase E R141Q	This study	RNase E (1-529) R141Q
RNase E R142Q	This study	RNase E (1-529) R142Q
RNase E R373K	This study	RNase E (1-529) R373K
RNase E R373Q	This study	RNase E (1-529) R373Q
RNase E R169K	This study	RNase E (1-529) R169K
RNase E T170V	This study	RNase E (1-529) T170V
RNase E (1-850) R3H, Q22D, H268S, Y269F, Q270D, K433N, R488Q, R490Q	This study	RNase E (1-850)
RNase E R3H, Q22D, H268S, Y269F, Q270D, K433N, R488Q, R490Q	This study	RNase E
Deposited Data
Crystallographic data and model, RNase E/MicC	This study	PDB: 5F6C
Crystallographic data and model, RNase E/RprA	This study	PDB: 6G63
Images of gels, Mendeley Data	This study	https://doi.org/10.17632/crmm6ccgy4.1
Oligonucleotides
GTTTTTTTTTTAATACGACTCACTATTACGGTTATAA ATCAACACATTG	This study	RprA forward
AAAAAAAAGCCCATCGTAGGAG	This study	RprA reverse
GTTTTTAATACGACTCACTATAGAAGCTGTTTTGGC GGATGAGAG	This study	9S forward
CGAAAGGCCCAGTCTTTCGACTGAGC	This study	9S reverse
MicC seed region connected through a tetranucleotide loop with a fragment of the ompD target encompassing the seed complementary sequence and the RNase E cleavage site GUUAUAUGCCUUCUUGGAGGUAUAUA ACAAAGmAmC	This study	MicC-ompD fusion
GUUAUAUGCCUU	Bandyra et al., 2012	12-mer MicC seed
ttttctcgagttaatacgactcactatagGCCATTGACAAACGCC TCGTTTAACAATGG	Bandyra et al., 2012	ompD forward
CGTGAACTTTACCGTACAGATCCAGTTTATTGCCG	Bandyra et al., 2012	ompD reverse
GCAGCAGGCGUUGUAAAUGCAGCCGAGGUAUAUA ACAAAGACGGCAAUAA	This study	50-mer ompD
GGUUAUAAAUCAACACAUUGAUUUAUAAGGUCAAA UAAUAAAGCCCGUCUCCUACGAUGGGC	This study	62-mer
Recombinant DNA
Expression plasmid for RNase E 1-529 wild type	Callaghan et al., 2005	pRNE529-N
Expression plasmid for RNase E 1-510 wild type	This study	pRNE510-N
Expression plasmid for RNase E 1-529 R3Q, Q22D, H268S, Y269F, Q270D, K433N, R488Q, R490Q	This study	pRNE529 8x mutant
Expression plasmid for RNase E (1-529) D26N, D28N, D338N	This study	pRNE529 D26N, D28N, D338N
Expression plasmid for RNase E R141Q	This study	pRNE529 R141Q
Expression plasmid for RNase E R142Q	This study	pRNE529 R142Q
Expression plasmid for RNase E R373Q	This study	pRNE529 R373Q
Expression plasmid for RNase E R169K	This study	pRNE529 R169K
Expression plasmid for RNase E T170V	This study	pRNE529 T170V
Expression plasmid for RNase E 1-850 R3H, Q22D, H268S, Y269F, Q270D, K433N, R488Q, R490Q	This study	pRNE850 8x mutant
Expression plasmid for RNase E R3H, Q22D, H268S, Y269F, Q270D, K433N, R488Q, R490Q	This study	pRNE 8x mutant
Software and Algorithms
CCP4 crystallographic suite	Winn et al. (2011)	CCP4
PHENIX	Adams et al. (2010)	PHENIX
Profit	Quantum Soft, Switzerland	Profit
Octet Data Analysis software	ForteBio	Octet Data Analysis
GeneSnap and GeneTools	Syngene	GeneSnap, GeneTools
PyMOL	DeLano Scientific	PyMOL
WinCoot	Emsley et al. (2010)	WinCoot
ISOLDE	Croll (2018)	ISOLDE