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. 2018 Oct 25;8:15792. doi: 10.1038/s41598-018-34094-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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WNT7A is overexpressed in PDAC cell lines and interference of WNT7A expression inhibits the migration capacity of pancreatic cancer cells. (A) The mRNA and protein levels of WNT7A were detected in five different PDAC cell lines and a nonmalignant cell line hTERT-HPNE by qRT-PCR and Western blot respectively. (B) The expression of WNT7A at mRNA and protein level were determined by qRT-PCR and Western blot respectively in cells transfected with negative control and wnt7a shRNA plasmid. (C) Impact of WNT7A expression interference on two PDAC cell lines’ migration capacity in transwell assays. (D) Impact of WNT7A expression interference on two PDAC cell in wound healing assays. ^**P < 0.01, ^***P < 0.001.