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. 2018 Oct 25;8:15792. doi: 10.1038/s41598-018-34094-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of WNT7A is up-regulated in hypoxia condition. (A) GSEA showed the relationship between WNT7A expression and gene set of hallmark hypoxia. (B) The protein expression levels of WNT7A in PDAC cell lines cultured in hypoxia for different duration were detected by western blot. (C) The knockdown efficiency of HIF1α in PDAC cell lines transfected with interfere plasmid were deteremined by qRT-PCR. (D) The effect of of HIF1α knockdown on expression of WNT7A in two PDAC cell cultured in hypoxia condition were detected by Western blot.