Table 2.
List of strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strain | ||
| E. coli C600 | F-tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 λ- | Lab collection |
| C600-GroE− | E. coli C600 ∆groel/groes | This study |
| C600-GroE+ | E. coli C600 harboring pGroE | This study |
| wt | E. coli C600 harboring pBV-TRAIL | Lab collection |
| GroEL/ES+ | E. coli C600-GroE+ harboring pBV-TRAIL | This study |
| GroEL/ES− | E. coli C600-GroE− harboring pBV-TRAIL | This study |
| Plasmid | ||
| pBV-TRAIL | Resistance to ampicillin, pUC ori | Lab collection |
| pGroE | Resistance to kanamycin, pMB1 ori | This study |