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. 2018 Oct 25;8:15752. doi: 10.1038/s41598-018-33982-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bone marrow stromal cells induce Mcl-1 expression in AML cells. (A) Expression of anti-apoptotic Bcl-2 family proteins in AML cells cultured on HS-5 BMSCs. OCI-AML2, OCI-AML3, HL60, ML-1, Molm-13 cells were cultured alone or in direct contact with HS-5 cells for 24 h and changes in the expression of Bcl-2, Bcl-X_L and Mcl-1 were determined with Western blotting. Levels of β-actin were measured as loading control. (B) Expression of Mcl-1 protein in the bone marrow- and peripheral blood-derived AML blasts. Whole cell lysates of mononuclear cells isolated from paired bone marrow aspirates and peripheral blood from 12 AML patients were analysed for the expression of Mcl-1 by Western blotting and quantified with densitometry. The left hand graph shows the expression values of individual patients and the right hand graph shows the value distribution as a box-plot. The p-value was determined using a paired t-test. (C) Mcl-1 expression in AML blasts cultured alone or with BMSCs. Mcl-1 protein expression was detected in bone marrow- and peripheral blood-derived MNCs from 4 AML patient cultured alone or on iMSC BMSCs for 24 h. Levels of β-actin were measured as a loading control.