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. 2018 Oct 25;8:15752. doi: 10.1038/s41598-018-33982-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of Bcl-2 and Bcl-X_L fails to revert BMSC-driven drug resistance. OCI-AML2 (A), OCI-AML3 (B), ML-1 (C) and Molm-13 (D) cells were cultured on a HS-5 BMSC layer for 24 h before exposing the cells to a combination of AraC and ABT-737 at the doses indicated (ABT3 = 3 nM, ABT10 = 10 nM, ABT30 = 30 nM and ABT300 = 300 nM) for an additional 24 h. The graphs show the average percentage of live cells ± stdev quantified with Annexin V staining from three independent experiments.