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. 2018 Oct 25;8:15824. doi: 10.1038/s41598-018-33892-z

Figure 3.

Figure 3

XOL-transduced cells were successfully converted into dOBs that produced calcified bone matrix in fibronectin-coated NanoCliP-FD gel. (a) RNA was extracted from the HDFs or XOL-transduced cells cultured in fibronectin-coated NanoCliP-FD gel for 14 and 28 days. mRNA levels for the indicated genes were evaluated by real time-RT-PCR. Values are means ± SD. N = 3. **p < 0.01 vs. HDFs. (b,c) Fibronectin-coated NanoCliP-FD gel with HDFs and XOL-transduced cells were cultured for the indicated days and stained with Alizarin red S. Some aliquots of the fibronectin-coated NanoCliP-FD gel were cultured without cell seeding (−). Macroscopic images (b) and relative staining intensities (c) are shown. Values are means ± SD. N = 3. **p < 0.01 vs. cell-free control. (d) Fibronectin-coated NanoCliP-FD gel with HDFs or XOL-transduced cells was cultured as above, and osteoimage assay was performed 21 days later. Confocal LSM images at low (upper) and high (lower) magnifications are shown.