Fig. 1. circ_104075 was upregulated in HCC.

a Venn diagram showing overlapped circRNAs that were highly expressed (log2 FC > 1) in HCC in studies performed by Huang et al., Fu et al., and Han et al., respectively. b circ_104075 expression was measured in ten pairs of HCC and adjacent normal tissues using qPCR. c circ_104075 expression was measured in normal hepatocytes (THLE-3 and HL-7702) and established HCC cell lines (Bel-7402, Bel-7404, SMMC-7721, HepG2, Hep1 and Huh7) using qPCR. d RNA expression of circ_104075, DANCR, HULC, UCA1, miR-21 and miR-223 was measured in the serum of healthy individuals (n = 60) and HCC patients (n = 101) using qPCR. The data are presented as the means + SD from three biological replicates. **p < 0.01, ***p < 0.001. The data shown in b, c, and d were analyzed using a one-way ANOVA test