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. 2018 Oct 25;9(11):1094. doi: 10.1038/s41419-018-1130-8

Fig. 7. Mitosis checkpoint protein Wee1 protects keratinocytes from UV-induced apoptosis and allows UV-induced squamous differentiation.

Fig. 7

Analyses of primary human keratinocytes 3 days after infections with the empty vector (CT) or specific shRNA to Wee1 (shwee1) and 24 h (a), 16 h (b, e), or 72 h (c, d) after sbUV irradiation as indicated. a Expression of Wee1 by western blotting or by RT-qPCR. GAPDH as loading control. b Expression of pro-apoptotic markers APAF-1 or BAX by RT-qPCR. c, d Analyses of shedding cells collected from the culture supernatant. c Representative flow-cytometry of DNA content. Bar histogram shows the percent of apoptotic cells in the sub-G1 fraction. d Representative flow-cytometry for the expression of keratin K1. Bar histogram displays positive cells relative to NI control (black broken line). e Expression of differentiation markers K1, filaggrin, involucrin (Invol) or loricrin by RT-qPCR. NI: non-irradiated. *p < 0.05, **p < 0.01. Data are representative or mean ± s.e.m. of triplicate samples. See also Supplementary Figure 7