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. 2018 Oct 25;8:15757. doi: 10.1038/s41598-018-34113-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adipose tissue dysfunction is associated with reduced lipogenesis and low adipose tissue PAHSA levels. (A) Relative quantification of GLUT4 mRNA measured in adipose tissue in relation to the lipogenic transcription factor ChREBP and its target genes ACACA and FASN. (B) Correlations of relative protein expression of GLUT4 determined by WB in isolated adipocytes in relation to different adipose tissue PAHSA isomers. Filled circles: subjects assigned to the IS group; filled squares: subjects assigned to the IR group. (C) Relative mRNA expression of the lipogenic enzymes ACACA and FASN in adipose tissue in relation to serum levels 9-PAHSA. (D) Relative mRNA expression of the lipogenic enzymes ACACA and FASN in adipose tissue in relation to serum levels of total-PAHSA (E) Correlations of adipocyte cell size and different adipose tissue PAHSA isomers. Filled circles: subjects assigned to the IS group; filled squares: subjects assigned to the IR group. (F) Correlation of GLUT4 protein expression and adipocyte cell size.