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. 2018 Oct 25;8:15768. doi: 10.1038/s41598-018-34021-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HLF gene expression following co-culture with healthy or asthmatic BECs. Expression of α-SMA (A), COL1A1 (B), and HAS2 (C) by HLFs co-cultured with healthy BECs (open circles, n = 15) or asthmatic BECs (closed triangles, n = 23) presented as scattered plots with lines and error bars representing mean and standard deviation if data was normally distributed. (A) Or median with interquartile range if data was not normally distributed. (B,C) To compare expression of genes between healthy and asthmatic HLF-BEC co-cultures, unpaired t tests were used for normally distributed data, and Mann-Whitney test was used for non-normally distributed data.