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. 2018 Oct 25;8:15768. doi: 10.1038/s41598-018-34021-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation between percentage of HLFs expressing cytoskeletal α-SMA following co-culture with healthy or asthmatic BECs and lung function measures of BEC donors. Percentage (%) of HLFs expressing cytosketal α-SMA quantified by flow cytometry following co-culture with healthy (n = 8, open circles) and asthmatic (n = 10, open triangles) BECs. (A) Representative immunohistochemistry (IHC) image of cytoskeletal α-SMA staining of HLFs co-cutured with healthy BECs. (B) Representative IHC image of cytoskeletal α-SMA staining of HLFs co-cutured with asthmatic BECs. (C) Correlations between % HLFs expressing cytosketal α-SMA quantified by flow cytometry following co-culture with asthmatic BECs and the lung function measures FEV₁% predicted (D), FEF_25–75% predicted (E), and FEV₁/FVC ratio. (F) of asthmatic BEC donors were calculated using the Pearson correlation coefficient.