Figure 1.

Low and high immunostaining of Lgr5, SOX2, CD8, Foxp3 and PD-L1 with corresponding digital mark-up of high immunostaining images. Representative images (70X) of each marker where blue represents negative staining, yellow weak, orange moderate and red strong staining in digital mark-up images. Scale bars=300 µm. Lgr5, Leucine-rich repeat-containing G-protein coupled receptor-5; SOX2, sex determining region Y-box 2; CD8, Cluster of differentiation 8; Foxp3, Forkhead box P3; PD-L1, Programmed death-ligand 1.