Figure 4.

miR-143 regulates GOLM1 expression via targeting its 3′UTR. (A) According to the TargetScan, the miR-143 target sites in the sequence of GOLM1 were predicted. (B and C) The fluorescence activity of the GOLM1 3′UTR was measured by the luciferase reporter gene assay in cervical cancer cells that were co-transfected with wild-type or mutational type GOLM1 3′UTR and miR-143, respectively (**P<0.01). (D) Western blot analysis detected the GOLM1 protein expression in cervical cancer cells with transfection of miR-143 mimics or inhibitor. GOLM1, golgi membrane protein 1.