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. 2018 Sep 21;16(5):6735–6741. doi: 10.3892/ol.2018.9478

Figure 5.

Figure 5.

Knockdown of GPR4 reduced the acidosis-induced cell apoptosis of HK-2 cells and HUVECs. (A) Representative images of TUNEL staining and quantification of TUNEL-positive HK-2 cells. Scale bar=20 µm. (B) Percentage of LDH release in the supernatant of HK-2 cells (expressed as a percentage of the maximal value). (C) Representative images of TUNEL staining and quantification of TUNEL-positive HUVECs. Scale bar=20 µm. (D) LDH release into the supernatant of HUVECs (expressed as a percentage of the maximal value). *P<0.05 vs. control group under normal conditions. #P<0.05 vs. control group under acidic conditions. GPR4, G protein-coupled receptor 4; LDH, lactate dehydrogenase; HCl, hydrochloric acid; HUVECs, human umbilical vein endothelial cells; si, small interfering RNA.