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letter
. 2018 Oct 11;141(11):e78. doi: 10.1093/brain/awy258

Figure 2.

Figure 2

PLD3 expression in the cerebellum is low and PLD3 KO mice do not show any signs of cerebellar degeneration. (A) Immunoblot analysis of PLD3 expression in different brain regions of adult wild-type mice with an antibody against the N-terminus of PLD3; n = 3. (B) Targeting vector for PLD3 LacZ reporter and PLD3 KO mice. (C) β-Galactosidase activity in different brain regions of an adult PLD3-reporter mouse with X-Gal as colorimetric substrate. (D) Immunoblot analysis of brain lysates with an antibody against the N-terminus of PLD3. (E) Photograph of the brain of wild-type and PLD3 KO mice, 20 months old. (F) Calbindin-immunofluorescence of the cerebellum of a wild-type and a PLD3 KO mouse, 20 months old. (G) Immunofluorescence staining for calbindin (red), GFAP (green) of the molecular layer in lobule IV of 20-month-old wild-type and PLD3 KO mice. Nuclei are stained with DAPI (blue). (H) Rotarod latency in 5- and 9-month-old animals (n = 9–11).