Figure 5.

Approach 1: Tissue classification based on raw, non-normalized SERS signal intensity. (a) Photograph and overlays of unmixed s420-CA9, s421-IgG4, and s440-CD47 images. (b) Stem plot of the non-normalized signal in each channel for all N = 15 samples. (c) Statistical comparison of the binding levels of s421-IgG4 in normal and tumor samples. (d) ROC plot for tissue classification using either all three channels (3-plex) or only the s421-IgG4 channel (passive). (e) Histological analysis of the data point indicated by a black asterisk in frame (b). Blue is DAPI nuclear stain, and red is a CA9 stain.