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. 2018 Sep 4;8(19):9590–9599. doi: 10.1002/ece3.4239

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© 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Genomic P elements in Drosophila simulans in Japan. The primer set of P176F and P2812R was used for PCR. The expected size of the full‐sized P elements is indicated as 2.6 kb. The samples were applied from left to right: SPP (02): ‐7, ‐8, ‐28, ‐29, and ‐30; OGS (03): ‐C27, ‐C30, ‐C31, ‐H3, and ‐H4; KMM (04): ‐1 and ‐2; OEB (05); AM06 (06): ‐3 and ‐4; TSM (08): ‐3, ‐27, ‐30, ‐31, ‐32, ‐36, ‐39, ‐58, ‐59, and ‐60; TN (10): ‐134, ‐136, and ‐140; MSO12: ten lines from ‐1 to ‐10; FWU12: ‐4, ‐6, and ‐7; Hikone15: ‐1 to ‐5, ‐7 to ‐11, and ‐13 to ‐21; and RM1 established in 1976. Harwich and Canton S were the standard P and M strains of the P‐M system of D. melanogaster. m: 1 kb DNA ladder marker (Takara)