Figure 5.

Sustainability of Exon 53 Skipping Induced by NS-065/NCNP-01 after Transfection for Short Incubation Times

(A) Schedule of cell differentiation, PMO transfection, and RT-PCR. (B) Exon 53 skipping induced by NS-065/NCNP-01 was measured in cells derived from a patient with deletion of exons 45–52 at 1 week after the start of transfection. NS-065/NCNP-01 was transfected for the indicated times. The bars show the mean and SD (n = 4). ***p < 0.001 compared to 0 μmol/L by Dunnett’s multiple comparison test. PMO, phosphorodiamidate morpholino oligomer.