Fig. 7.

The proliferation of monocytes (THP-1) and HUVEC evaluated by the MTS assay. After 24 h and as compared to untreated THP-1, the proliferation of THP-1 in the presence of H₂O₂ and DBMSCs (THP-1/DBMSC + H₂O₂) significantly increased at different THP-1 and DBMSC ratios (20:1, 10:1, 5:1, and 2.5:1) (a). After 24 h and as compared with untreated HUVEC, the proliferation of H₂O₂-treated HUVEC (HUVEC + H₂O₂) and H₂O₂-treated HUVEC in the presence of THP-1 pretreated with H₂O₂ (THP-1 + H₂O₂) at 1:10 HUVEC:THP-1 ratio significantly increased (b). The proliferation of H₂O₂-treated HUVEC in the presence of THP-1 pretreated with H₂O₂ and DBMSCs (THP-1/DBMSC + H₂O₂) significantly reduced as compared with H₂O₂-treated HUVEC cultured with THP-1 pretreated with H₂O₂ (b). After 24 h culture with DBMSCs and as compared to monocytes cultured alone, the proliferation of THP-1 significantly increased at all examined ratios of monocytes and DBMSCs (a). As compared to monocytes cultured with H₂O₂ alone, the proliferation of monocyte cultured with DBMSCs did not significantly change, P > 0.05 (a). Each experiment was performed in triplicate and repeated for five times with five independent preparations of DBMSCs and HUVEC. *P < 0.05. Bars represent standard errors