Table 2.
DBMSCs modulate the expression of genes involved in endothelial cell (EC) survival, apoptosis, injury, fibrosis formation, and inflammation. THUVEC (HUVEC were cultured with 100 μM H2O2 for 48 h). TDBMSC (HUVEC were cultured with DBMSC and 100 μM H2O2 for 48 h)
| # | Gene symbol | Gene full name | THUVEC mean ΔΔ−2 values | TDBMSC Mean ΔΔ−2 values | Fold change (TDBMSC Vs. THUVEC) P < 0.05 |
Biological activities |
|---|---|---|---|---|---|---|
| 1 | BCL2 | B-cell Lymphoma 2 | 2 | 23 | 11.5-fold ↑ | Induce EC survival |
| 2 | EDN1 | Endothelin-1 | 12 | 82 | > 6.64-fold ↑ | |
| 3 | EDNRA | Endothelin-1 (ET-1) Receptor A | 3 | 9 | 3-fold ↑ | |
| 4 | HMOX1 | Heme Oxygenase-1 | 7 | 158 | 20-fold ↑ | |
| 5 | KDR | Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) | 2 | 52 | 22.57-fold ↑ | |
| 6 | MMP2 | Matrix Metallopeptidase 2 | 9 | 36 | 4-fold ↑ | |
| 7 | SPHK1 | Sphingosine Kinase 1 | 44 | 840,958 | 19112.68-fold ↑ | |
| 8 | MMP9 | Matrix Metallopeptidase 9 | 1 | 6 | 6-fold ↑ | |
| 9 | PECAM1 | Platelet endothelial cell adhesion molecule | 1090 | 7242 | 6.64-fold ↑ | |
| 10 | TNFSF10 | TNF-related apoptosis-inducing ligand (TRAIL) | 7 | 30 | > 4.20-fold ↑ | |
| 11 | TYMP | Thymidine Phosphorylase | 5 | 27 | > 5-fold ↑ | |
| 12 | FAS | Fas cell surface death receptor | 3 | 0.76 | > 3-fold ↓ | Induce EC apoptosis |
| 13 | PF4 | Platelet Factor 4 | 21 | 0.69 | > 30-fold ↓ | |
| 14 | HIF1α | Hypoxia-Inducible Factor-1α | 159 | 11 | > 14-fold ↓ | Induce EC injury |
| 15 | SERPINE1 (PAI-1) | Type 1 plasminogen activator inhibitor | 997 | 248 | 4-fold ↓ | |
| 16 | TIMP1 | TIMP Metallopeptidase Inhibitor 1 | 3 | 1 | 3-fold ↓ | |
| 17 | ACE | Angiotensin I Converting Enzyme | 15,765 | 5 | 3153-fold ↓ | |
| 18 | F2R | Coagulation Factor II Thrombin Receptor | 20 | 1 | 20-fold ↓ | Induce EC inflammation |
| 19 | ADAM17 | A disintegrin and Metalloprotease 17 | 256 | 4 | 64-fold ↓ | |
| 20 | FLT1 | Vascular Endothelial Growth Factor Receptor 1 | 23 | 12 | 1.91-fold ↓ | |
| 21 | TNF-α | Tumor Necrosis Factor-α | 591 | 2 | > 295-fold ↓ | |
| 22 | PTK2 | PTK2 protein tyrosine kinase 2 (PTK2) or focal adhesion kinase (FAK), | 8 | 2 | 4-fold ↓ | Inhibits inflammation and fibrosis |
| 23 | PLG | Plasminogen | 8 | 90 | > 11-fold ↑ | Inhibits inflammation and, promotes fibrin clearance |
| 24 | F3 | Coagulation Factor III, Tissue Factor | 30 | 0.06 | 500-fold ↓ | Induces EC injury by induction of fibrin and thrombus formation |
| 25 | THBD | Thrombomodulin | 1013 | 1 | 1013-fold ↓ | |
| 26 | THBS1 | Thrombospondin 1 (TSP-1) | 1097 | 3 | > 365-fold ↓ | Induces EC inflammation and injury |
| 27 | TFPI | Tissue Factor Pathway Inhibitor | 11 | 23 | ~ 2-fold ↑ | Inhibits EC injury by reducing thrombus formation |